Proteomic Variability and Nutrient-Related Proteins across Pigmented and Non-Pigmented Rice Grains

Rice protein is considered an important dietary source. Information regarding rice nutrient-related expression still scarce, hence further study investigating this aspect highly needed. Herein, we applied sequential window acquisition of all theoretical mass spectra (SWATH-MS) for a comparative proteomic analysis across six different Malaysian varieties. These consisted black (BR: PH9 and BALI), red (RR: MRQ100 MRM16), white (WR: MRQ76 MR297). This aimed to unravel proteins if their expressions were significantly A total 4022 quantified found be expressed varieties with false discovery rate (FDR) < 1% p 0.05. While among 1792 differentially (DEPs) that identified, 74 DEPs had functions related nutrient biosynthesis. There higher key enzymes the carotenoid amylopectin biosynthesis pathways seed storage proteins, i.e., prolamins glutelins in RR. Glycoproteins such as cupin germin-like protein, well are involved thiamine anthocyanin abundantly BR. WR was particularly enriched essential amino acids (methionine arginine), vitamin B, unsaturated fatty acid. provides us insights into differential functional properties shaping grain pigmentations plant immunity, contributing diverse health benefits daily food human consumption.